Luffy and crew takes on Arlong's crew in baseball. Announced by Bon Clay and Buggy. (Source: ANN)

Continuing where the first season left off, the sudden increase of Thanatos with the disappearance of the Aube Hunters has now left Arcadia in ruin. Desperation reigns high as the Religious Organization fights to battle the Thanatos. Six months had already past since Hidamari mansion burned to ashes, the Aube Hunters, once hailed as heroes, is now blamed for the appearance of Thanatos. Nevertheless, hope still exists for the reappearance of the Queen's Egg one day, who will become the savior of Arcadia. Unknown to many however, the Aube Hunters have actually been silently waiting for the awakening of Angelique, who has been asleep the entire time.

Prior to the events of the anime, three factions—the Garan Army, the Kiba Army, and the Aira Army, battle for total dominance of Machine Island. The island is basically a prison, isolated from the rest of the world. The island contains a vast amount of resources, allowing the three factions to create an infinite supply of giant robots for their respective armies. The world government learns that the island's power reactor has become unstable and will explode within 66 hours, destroying all of life and Earth itself. The government sends the Death Caprice squad, composed of mercenaries Ken Kaido and Ryo Magami to solve the issue, along with their giant robot Mazinkaiser, but was that a good idea?

In search of true love, Kanako Miyamae transfers to Ame no Kisaki Catholic school, inspired by how her parents fell in love with each other there. There is just one difference, though: because men make Kanako break into hives, she has actually come to the all-girls school to find a partner of the same sex. When she meets the beautiful Mariya Shidou, Kanako believes she has found that special someone; however, there's more to Mariya than meets the eye—it turns out that Kanako's first love is actually a cross-dressing boy. Mariya threatens to expose Kanako's impure intentions unless she keeps his real gender a secret, and to make things worse, he also replaces her original roommate so that he can now keep a close eye on her. Maria†Holic follows Kanako as she looks for love in all the wrong places and searches for the girl of her dreams—that is, if she can survive being Mariya's roommate! [Written by MAL Rewrite]